FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605311
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT12605.456968E-1211.5892261
CTCAGCT12801.19162E-89.6969231
CTTAGAT9352.0009478E-59.370541
CTCTAAG7050.00132590729.3206961
CTTTTAT62950.07.65496441
CTCAGAT16356.177979E-77.59147451
CTCCAAT22356.4210326E-107.51355171
CTCGTAC9750.0029200467.48842331
CTCTAAT10850.00101136657.402151
CTTAGCA10900.00106206027.3681961
CTCAAAT24851.03682396E-107.34528351
CTTAGAA14053.810019E-57.2752291
CTTCAAC31350.07.21969941
CTTTGAT49600.07.2128911
CTTGGAT18304.780941E-77.18152051
CCCGAAT16505.7325506E-67.07996271
CTTGAAT29953.6379788E-127.06962161
CTCCAAC19652.0737752E-77.0596961
CTTAAAT29009.094947E-127.0494461
CTCCTAA11400.00170880827.0450291
Produced by FastQC (version 0.11.5)