FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823326
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT2650.002425257116.0156333
TCACGGC7950.00523911378.0078162
CAAATCT9804.5277207E-47.97848841
AAACTTA9450.00292875137.48480565
CTTTAAA13352.2196247E-46.9217531
CAAAGAG10300.00645731766.9010761
CAATTTC13703.233744E-46.71214774
CTTTTAT18456.2729287E-66.54947661
CTTTCAA14606.605416E-46.3291371
ATTCTGT12650.00706916076.15055756
GTTCCAT12950.0085620096.0377751
AAGATTT19107.793685E-55.92550473
AAAGATT19506.5569463E-45.44120842
AAACAAT17200.00467471035.3724081
CAAAAGC17300.00500459745.3413531