FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359578
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGT4754.9411564E-412.386931
GTCTAAT6908.6220396E-510.6590421
CCTACAC9552.1705728E-610.0116871
ACACGTC5950.00381644869.6958938
CACGTCT6600.0089717398.7387719
CCCTCAG6800.0097288138.6526351
CTCAAAT20851.2551027E-108.1131421
CCCAGAT19101.6007107E-98.0863631
CTTCGTT18306.15546E-98.0379671
CCCCGAA14658.6191176E-78.032481
CCCCAAT8250.0051557668.0233521
CCTCAAT18356.4774213E-98.0160651
CTCACGG14054.0965406E-67.85203551
CCTACAG8450.0063927197.83345031
CTATGAT24104.3655746E-117.6293981
CTCTAAT10808.90671E-47.49093871
CTTCACT15752.5649242E-67.4714821
CTCCCAT15051.0839667E-57.33030561
CCCCGCT12153.8929295E-47.26394031
CTCAAAA33500.07.24496751
Produced by FastQC (version 0.11.5)