FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732566
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2406.162372E-826.751179
CACACGT2654.346475E-621.5355327
ACACGTC2909.48126E-619.679028
AGCACAC4702.2738004E-615.1779685
GCACACG4252.4957844E-413.4280396
GAGCACA5300.001596088510.7677664
AGAGCAC5500.002172529210.3762113
CGAATAT7954.870318E-48.9731383
AAGAGCA10551.07814994E-48.1152072
CCAAGAA7950.00478012638.0896291
CTTCCAT10307.2871085E-47.6314791
GAAGAGC8500.00870708757.56618261
CTTCTCT8600.0096649727.4782041
Produced by FastQC (version 0.11.5)