FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639066
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2458.677489E-825.8216297
CACGTCT2601.5431215E-724.3300179
ACACGTC3351.7653565E-618.8844748
AGAGCAC3907.5211756E-616.2212813
AGCACAC4602.1183914E-615.2809165
GAGCACA4853.6706697E-614.4932394
GCACACG4452.6212883E-514.2164036
CGATTGG3100.006665162713.7556331
TTCCGTG6450.0091061338.7211442
GAAGAGC8700.0012375488.1690541
AAGAGCA9400.00294411747.48023652
CAACAAA10350.00677175736.86674071
TTGATTT14100.0034956495.9823163
Produced by FastQC (version 0.11.5)