FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115915
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG5500.001966509510.502179
CTTATGT6707.7382504E-49.8844021
CTCAATT7200.0014948849.1979861
TGAATCC8900.00132551028.1137223
CAAGAGA8450.00635896957.83733651
CTCCAAT8750.0086888777.5686291
CTCATCG14353.5204928E-46.6661361
CAATAAT15751.534862E-46.540791
CACAAAA17005.8915703E-56.4926961
GGGGGGG2850.00291990236.081565495-99
CATAAAT14950.00403778075.9063991
TCAAAGG15250.00619732965.68277452
CTTCATC23156.914334E-45.08571151
CGAAGAA19000.009998755.0346871
Produced by FastQC (version 0.11.5)