FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693521
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGG9351.814899E-59.4476611
GTTCTAA6300.00515922959.3476871
CTCACAC6750.0090901338.7245071
TCCTAAT8659.953286E-48.3525953
CTCAAAT14356.2095023E-78.2077251
TCCCGTA8500.007918267.64818769
CTTTAAT19151.1427619E-77.30364271
CCCATGA13058.713838E-46.76901441
CTTTAAA21801.5198384E-76.7534891
CTTCAGT13300.0010808916.64177751
CCTGGAT12700.0048698766.3759321
CCTCAAT13950.00185321536.3323041
CTCAAAA22351.6659269E-66.25793081
CAACAAG28601.3764293E-86.1773171
CTCAGGA14350.00254597036.15579371
CTCAAAC13300.0078664586.0882961
CTTTCAA27803.20877E-75.8254921
CACAGAA21955.3180862E-55.7012371
CAACGAA15650.0066841225.644451
GATTTTG32103.9197403E-85.6269435
Produced by FastQC (version 0.11.5)