FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728130
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2258.258212E-1032.0946127
ACACGTC2705.798938E-926.745518
CACGTCT3453.105015E-923.0212359
GCACACG4054.2283318E-717.830346
AGAGCAC4701.0806434E-716.9008873
GAGCACA4307.9176607E-716.7936924
AGCACAC4752.2355634E-615.2027115
CTCTTGA5950.00344665639.8144491
CTCATCG6200.00484170769.4187051
CAAAGTC7050.00146334789.2186664
CTTACCG6550.0076045258.9154161
ACAAAGT7550.00272790658.6081583
GAAGAGC8557.9961034E-48.5374231
CAATGTT8150.0049359998.0607941
ATGATTC10055.0059985E-47.90389733
CCATTAT9550.00238371457.64345171
CTTTGAT13051.2094171E-47.2715291
AAGAGCA11150.00150914337.1265882
TGATTCT11400.00191483776.9679094
ATTTGAT11850.00257034586.7759041
Produced by FastQC (version 0.11.5)