FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484129
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT11653.5215635E-910.4453581
CCCAAGA13202.6706402E-78.6765371
CTCACTT15806.2209438E-98.6078931
CTCTCGT13754.9737537E-78.3294751
CTCACAG15353.0991578E-77.92758461
CCTAAAC8250.00657695537.80888371
CTCAAGT18601.1223892E-77.3120811
CCCAAGT10850.00124840967.25710341
CCCATGA24801.5643309E-107.215871
CCCAGTT24156.6029315E-107.11368231
CTCAAAT29552.5465852E-116.78267341
CTTTGAT59100.06.78267341
CCCAAAC14901.0695395E-46.72577241
CTTCAAT55000.06.6375511
CTATGTA16304.8475846E-56.5860194
CCTAAAT17601.9964402E-56.50740341
CTTTAAT52000.06.469861
CTCGCAC12250.00447922066.427721
CTCAAAG35851.8189894E-126.3894161
CTCACTC12450.0053029766.32446341
Produced by FastQC (version 0.11.5)