FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806337
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCC3153.6770827E-416.010046
CTTCAAT16207.2759576E-129.870851
CTCCAAT6300.00572148069.2298851
CTTTCGT7903.7924515E-49.2006761
TTTGTAG7350.00218446258.8218598
CGATTTC11001.5645358E-47.85947474
CTTCAAG15351.6822984E-57.10280281
GGCCTTC3700.0094147536.7043366145
CAAAGGT12450.00451049076.42199851
CCTTCAA12550.0049030016.37082671
AACAAAT13750.00199646156.289532
CTCCTTT14700.00383344835.9334981
CTTTTAT17356.2581047E-45.86509941
CATTCTT17406.4925983E-45.8482461
TCCAGAT15000.0052766085.7654032
CAATAAA16800.0027187995.6244611
CTCTTTT18600.00151496095.470941
Produced by FastQC (version 0.11.5)