FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490668
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATT4100.002626113112.3419092
TTCATAG4350.004096077311.6302327
CTCAAAT4550.005662327611.1360491
CTTATAA6305.099217E-410.3406171
CCGATTT5950.00373489719.7184493
CTTCAAA9452.4992254E-59.191661
CGATTTC8700.00104604778.3081564
Produced by FastQC (version 0.11.5)