FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970147
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3505.3386488E-516.096167
CACGTCT4051.85163E-413.9102629
ACACGTC4353.3908803E-412.9509338
AGCACAC5200.001517347810.8339545
AATCAAC7251.8749546E-510.6845226
GCACACG5550.002608647610.1507326
AGAGCAC5650.00302230299.9721013
GAGCACA5850.00402992139.6311754
TCTCGCT7450.00303724068.5071997
ATATCAC8359.0358977E-48.4336186
CTCCAGA9302.7860096E-48.346971
ACAACAG9650.00374955367.29823454
ACAATAT12951.7121411E-47.06998873
CTGGATT10300.0069274086.85143951
CAATTTT12900.0090539076.0054854
GGAATTT15000.00681122255.6342373
GAATTTT15250.0081734355.54187254
GAATTTC15300.0084733845.5237624
Produced by FastQC (version 0.11.5)