FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203103
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT6900.035.170999
CACACGT7450.032.5744747
ACACGTC7300.032.2660528
AGAGCAC9550.024.658983
AGCACAC10300.023.559195
GCACACG11500.020.4802276
GAGCACA11650.020.2165344
AAGAGCA18050.013.0505132
GAAGAGC19750.012.3203621
CCCCAAT4500.005683494311.1326141
GTTTAAC4650.007252877610.7734991
CTCCAAG9252.0429034E-610.0580381
GCACTTA6200.08.74827830-34
GAGGTAA7550.00303658288.507745
CTCGTAT7800.07.5024935-39
TCGAAAT10600.00100545777.40544753
CCAGTCA7850.07.27324420-24
ACTCCAG7300.07.23403415-19
ACGTCTG7500.07.23196910-14
AGTCACC7700.06.85881120-24
Produced by FastQC (version 0.11.5)