FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675214
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC48050.08.8937413
CTTCCGA77750.07.94443661
CGATCTA15352.8315737E-56.84581665
CTTAAGT24808.7809894E-86.2266121
CCGATCT60900.06.211864
CCCGAAT46850.06.14264061
CCCCCGT19451.7365246E-56.13493731
CTCGAAT30402.2595486E-85.77227831
CCCAAGG34151.4351826E-95.75503731
CCCTGAG26954.440517E-75.72986941
CTCAGAT42901.8189894E-125.72653061
CCCACGT21306.9578244E-55.6020911
CTCAACT33503.2447133E-85.4476521
CCCCCAT25353.1226788E-55.26085661
CCTAAGA18750.00261068255.24092051
CTTGAAT71800.05.18122241
CTTCGAT77400.05.16909741
CTCCCAC42201.5770638E-95.15620371
CTTCAAT110750.05.07022331
Produced by FastQC (version 0.11.5)