FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707562
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAT2800.002954060215.5484591
GAGTCAG3250.00812162513.3510615
CTTCACA6304.9927266E-410.365641
CTTCGAT7753.1832926E-49.3625141
CTCCCGG7200.00169700839.0699351
CTCAAAG6700.0096128768.6638171
GTCATAT7650.00294242928.5364081
CTCATTT8950.0013345638.10720351
ATTTGTT14655.3612224E-46.43872451
ATCAAAT13850.00200542676.28674171
CTTTCAA13850.00200542676.28674171
CTCTTTT17205.722104E-45.90600351
AAGATAA17457.180502E-45.80203635
CTTTTCT18852.9383763E-45.77396351
AAAACTT15650.0081116375.54516844
Produced by FastQC (version 0.11.5)