FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522383
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5650.036.6279757
CACGTCT7150.028.9437879
ACACGTC7750.026.7029748
AGCACAC11800.019.3522435
AGAGCAC13800.016.0320383
GAGCACA14200.015.0778384
GCACACG13850.014.9420986
AAGAGCA22800.010.644062
GTCACTA4200.010.55281120-24
GAAGAGC21250.010.4639831
AGTCACT4600.09.63517420-24
GTTCGGA10105.684548E-47.81206041
CATTCAT11803.7074034E-47.29446551
ATCGAAT10200.00538585947.0322461
CTCGAAA12900.00102566746.67245671
GTTGAAT11900.00323417836.63040351
CTCGTAT7600.06.394754435-39
CTATTCG7352.5465852E-116.026964725-29
CACTATT7853.6379788E-126.00715125-29
CAGTCAC8007.2759576E-125.897659320-24
Produced by FastQC (version 0.11.5)