FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605825
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAGC6700.0097902668.64567851
CCAACGA11001.6677204E-58.5255498
CTCAAAA20701.5097612E-108.0452831
GTTTAAT13552.1275599E-46.94685031
CTCTTGA11550.00214041286.89595751
CTCAAAT14909.192992E-56.8033941
CAAACAT18605.353437E-66.6178951
CCCAAAT12250.00396998276.50190261
CTTTAAT23851.5382102E-76.3755081
CTCCATT19701.30558965E-56.24836731
AAGTAAG13950.00232448486.20591456
CTTTAAA22803.1039635E-66.03396271
CAAATCT17200.0037661775.47266341
CTTTGTT28554.346579E-65.325951
CACAAAG18100.00685836755.20054241
CAAAGAA47751.2369128E-105.1557211
CTTCCGT24002.6134652E-45.1288681
CTCCTTT32501.6049798E-65.1242271
CAAAGTT18600.009424535.06074331
Produced by FastQC (version 0.11.5)