FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294438
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA18255.8427577E-77.09561061
CTCAGAT14557.336118E-56.9222161
CTCGGAC13652.5226796E-46.8515811
CCTCGAT28808.367351E-116.74452451
GGTAGAT18555.687023E-66.5930311
CTCGTAA14204.0863504E-46.5862031
CTCAGGA16505.3405434E-56.5401461
CTCCAAT26753.6581696E-86.1856331
CTCACAT12800.0067141756.1824821
CTCGATT18252.1356103E-45.91300871
CTCGGAT15850.00154050355.90057331
CCTAAAT17105.9494836E-45.8899561
CCCAAAC18502.570378E-45.8331031
CCCGCGA20151.3275063E-45.7124851
CCCGAAT18903.436651E-45.70965051
CCCGCGT17850.00102825845.64247851
CAACGCT23052.632038E-55.6179991
CTTAAAC16650.00276990235.61706161
ATTCGAT27001.7612219E-65.5954581
CTCGCTT16850.00319095545.55039071
Produced by FastQC (version 0.11.5)