FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571281
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCCA350.00592307454.6662032
ATCTCGT5554.2242138E-420.766354145
CCCAAAT2007.108329E-419.2325531
CTCTGGA4300.00917255310.4362681
CGTAGAG2350.0041941039.585151135-139
CACAACC3702.7102378E-59.1318135-139
CAGTCAC4358.415314E-68.148742130-134
AGTCACA4955.681488E-67.761162145
AGATTCT7400.007004627.7519394
ACAACCG4755.9047336E-57.705957135-139
GTCACAA5251.3039806E-57.234087130-134
CTCCAGT4305.339883E-47.065852130-134
AACCGTT6050.00191986346.312024140-144
TCACAAC5000.00657390346.1944036135-139
CCAGTCA4450.00267743166.128163125-129
CGTTCAT5850.0094014235.983835140-144
TCATCTC7758.0942037E-45.748693140-144
Produced by FastQC (version 0.11.5)