FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837235
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACT6300.00623773749.1345671
CTCCGCT15654.431422E-88.2736431
ACACGTC7900.00449358678.1466968
CCCAGAT16353.345583E-46.1595481
CTCGAAA13300.0099844445.9494881
CCAAGAT14750.00447745765.85231641
CAACAAC24702.173214E-65.82467371
CTCAAAT15300.0067180625.6419381
CAAAGAT25955.19907E-65.54410171
TCCGGGG15600.0088566085.50225832
GTTTATT18850.00204816625.3426321
CTTTTAA23502.1059693E-45.20378831
AATTTAA19350.0030626415.17384245
CTTCCAC22707.227867E-45.07028871
CAGGAAT22807.688188E-45.0480511
Produced by FastQC (version 0.11.5)