FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516733
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATCTCGTATGCCGT5490.10624442410297TruSeq Adapter, Index 6 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1600.053.064649
ACACGTC2050.044.8676648
CACACGT2100.040.4341167
AGAGCAC3550.029.9042513
GCACACG3053.092282E-1127.8398846
AGCACAC3451.4551915E-1024.6144525
TTACGTG1450.001412852724.3975378
GAGCACA4258.367351E-1121.6483354
GAAGAGC6602.7901478E-813.997121
AAGAGCA7401.306762E-712.4391862
CTTAAAT4250.00390659811.7043791
TCAACAT5750.00335153569.8467343
GTTTGTT6950.00153512959.1693382
AGTCACT2653.024068E-68.021420-24
TATGATC3650.0097681467.75974462
ACGTCTG2501.0479247E-47.358439410-14
GTCACTC2551.3147797E-47.224502620-24
CAGTCAC3051.6524493E-46.50478620-24
CTCCAGT3051.6818341E-46.49597315-19
ACTCCAG2906.438607E-46.343972715-19
Produced by FastQC (version 0.11.5)