FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593211
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAAGA5458.278719E-613.3970271
TACTTGT3250.00867460413.2181315
CAAAGGG4950.00992953110.3251841
CATAAAT6200.0048290719.4211351
CAACAAA7853.3980524E-49.3011211
CTTTGAC7900.003717028.31802751
TAAATAT8150.00586866777.9065513
AATCTTT8650.0099962667.44952445
AAGAAAT12507.2973734E-46.8734283
Produced by FastQC (version 0.11.5)