FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158756
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAT6954.154208E-813.5833761
CCCAAAT7301.4404057E-510.9425611
CTTGTGT5550.00202130410.4675771
CACCTTA4900.00959748610.3741161
CTTCGTT9201.5806017E-610.2613541
CTCCCTT8557.3692445E-610.1921151
TCCACTC7900.00426553058.1934227
CTTCATT18005.7116267E-98.0687571
CCTCTAT9150.00164839327.93648241
CTTTAAT17502.9602234E-87.8843281
CTTATAA15151.7283292E-67.66931341
TCCGTCT10357.1664684E-47.64433533
CTCTTAT9550.0025103977.6040641
CTTCGAT11602.6408E-47.5122911
CATATGT9800.00323310357.4100831
CTCAGAA9900.0035701957.3352331
CTTAAAG12204.7145534E-47.14283471
ATTTCGT10450.0066348546.8823016
CTTCCGA10550.0072753286.8170667
CTTTGTT16053.1843956E-56.7868051
Produced by FastQC (version 0.11.5)