FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947445
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGAT5950.00345574239.8118921
CTCTGAT6758.9368154E-49.7301251
CCCGAAC6150.00454090549.49280551
CTCTTAT7200.00161184449.1219931
CTCCGAT7400.0020681778.8754521
CTCAACG10104.981209E-58.6704081
CCCAAAA8100.0046837498.10843851
CTCCAAA9500.0022713587.68167831
CTTATCT10607.917179E-47.5729751
CAAAAGG11702.7535428E-47.4847121
CTCATTG9800.00308039167.4465251
CTTGAAG16003.6369092E-67.2975941
CTTCCGT11100.00129291087.23185061
CTTTTGT14154.2001357E-57.22023441
CAACGGA10200.0045511317.1545051
CTGCCGA13952.756859E-46.80062531
CTTTCAT15601.5087391E-46.54912331
CTTTGAT13400.0012965726.5351591
CTTCATT15308.468654E-46.20057061
CTTCAAG16300.00181056175.8201671
Produced by FastQC (version 0.11.5)