FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166065
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA6506.8119064E-410.0226921
CTTAAAG9753.4605819E-69.6514821
CTTCGAA6650.0092277398.7081031
CTCCGCT9452.5179083E-48.4258961
CTCTTAT9250.00189358657.8255251
CTTTAAT19452.0092193E-77.0711361
ATGAACG12355.779058E-47.0168787
CTTTTAA22203.9592123E-86.84733441
CTGGAAT15751.9001446E-46.4343211
CTTGAAG13550.00161242886.4105781
CTTTGGA14756.0047564E-46.3797921
TGCAAAC12550.0052437846.3304482
CTTTCGG16102.5262055E-46.29444461
CTGCAAA26853.4619006E-86.2006721
CTAAAAA16854.542686E-46.0142761
CTTATAT16300.00199595515.77312561
CTAAAAT15050.00521907685.771651
CTCAAAA17607.929728E-45.75798561
CTTTCAT24107.969478E-65.70678851
CCAGAAT16500.00230794425.70314741
Produced by FastQC (version 0.11.5)