FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687323
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAA4050.002241600312.6061891
CCCAACG4250.003217504412.0129571
TTAATCT6300.00606696039.1639863
CCCGGAT8205.3157314E-48.894611
AAACACC6600.0088912288.7468046
CTTTGCT8550.00764060257.6774531
CCTGCAT10350.00526889137.0469381
CTTCAAT10550.0063438516.9133471
ATTTCAT14705.9723825E-46.38159756
AAGATAT15350.00100627186.11181163
GAAGATA14200.00281997686.0985682
CTTTTCC19700.00298408745.18325571
Produced by FastQC (version 0.11.5)