FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588608
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACT2550.002275396116.1682821
CTCAATT3100.00832890713.2997141
CTTTAAT6751.1126158E-511.1980311
CTTAAAA6306.805245E-510.9071731
TTTGATC5150.001736413210.6587842
CTTCAAC7906.3039624E-59.5679381
CTTTGAT9504.8080965E-69.40313151
CAATTAT6300.0090651758.7257391
CTTCAAT8802.0407903E-48.5893981
CAAAAGA10251.19210046E-48.0447051
GTTTGAT7800.00572311557.9286761
ATTTTCC14001.0368161E-57.3504566
CAGATTT9350.0035539247.33735373
CTTCAAA9700.0050043827.084041
CATCAAT11856.3418684E-46.95850041
TTTATTT17300.00755682355.156112
Produced by FastQC (version 0.11.5)