FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863942
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGA2700.00225011916.196571
TTACGGG3558.955513E-414.238528
CCCAATT4050.00225428712.5973331
AAACCCA4100.002650472812.3284744
CCCACTT4400.004193578411.5952721
CCACGTT4600.005841349711.0911291
CTTACCT7001.0916064E-410.4120811
CTACAAT8708.67062E-610.0530431
CCTACAT5850.0030342149.967121
CTTGTTA8456.920591E-59.4879311
CTTACGA6250.0052395499.3292241
CTTTAAT17202.419256E-108.8986971
GTTATCC6600.0088462888.7526835
CTCAAAA11001.4623291E-58.613631
CTTAATT9853.653526E-48.1393931
CCTCCAT8200.00528855257.99952551
ATCCAAT10155.04329E-47.898821
CTGGAAT9750.00296553957.475341
CCATGAT10150.00439087357.18074561
CTTCAAA17301.5460337E-67.1620671
Produced by FastQC (version 0.11.5)