FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123133
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACA4050.002189582712.6468191
CCGTAGG5501.4776672E-411.8225286
TAGAGAC4450.00487236911.3649783
CTCCAAT9051.161141E-610.5107331
CCCAAAT8405.4508746E-610.4529821
CTCAAAT11753.3884025E-68.7182321
CTTAACA7650.0027297078.6083381
CTTAAAT11954.2549636E-68.572321
AGTAGAT8100.00457396278.1300981
CTCGAAA9250.00170269917.91036561
CTCAAAG11401.9792069E-47.70219761
CTCCATT14305.647302E-67.67526671
CCCAGAA9700.00271528457.54338931
ACGAAAT10250.00526274557.0486623
CCTCTAT10450.00561318137.0019981
CCAAGGC10600.0072898526.815622
CTTATAT16454.0364805E-56.67211631
CTTTCTA13350.00120647266.57715751
CTTTAAT19056.5871172E-66.52968451
ATTGAAT14300.00261710176.14021351
Produced by FastQC (version 0.11.5)