FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5377637
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA20851.8189894E-128.6915081
CTTCTAT78550.07.10568141
CTTTAAT94800.07.03462031
CTCTAAC16304.062122E-56.6705991
CTCAAGT21309.52592E-76.4659921
CTCAAAT50900.06.4084931
CTTTAAA107950.06.379141
CTCAGGA33506.184564E-116.2750091
CTCTCCT38351.8189894E-126.23748871
CTTGTAT59500.06.21318671
CTTCAAT105050.06.21022941
CTCACAT24552.6070848E-76.20053241
CTTTGAA113550.06.0645371
CTTTGAT107050.06.02649071
CTCAAGG27706.398659E-86.01879071
CTCCCAC28058.259849E-85.943691
CTTTTAT144300.05.92757231
CTTGAGA30701.3833414E-85.90286451
CTTTGTT105000.05.86800961
CCCAGGT19859.59705E-55.8427951
Produced by FastQC (version 0.11.5)