FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462527
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGAG2952.1921533E-417.1309599
TCACGTT3300.00907928913.126322
TTAAACT4050.002418195612.4781063
CTTCGAT5351.11734305E-412.1791461
CTTCGGA4200.003109767812.0663761
CTCAGCG4450.004790559411.388491
CTTCCAT8709.359239E-69.9859671
CAACAAT6300.00590285159.1934291
CTACAGC6300.00590285159.1934291
CCTCTTC10500.00649921226.8950721
CTTTTTT14650.00384721325.9302321