FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354657
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTGT5700.00258614810.1618661
CTCTTAT10951.412991E-79.9182611
CTCAAAA19253.6379788E-129.0269051
CTCAGCA8659.7430556E-48.3703241
TTGATCT11101.711326E-47.7999143
CTTGAAG15302.0972038E-67.57158761
CTTTGAT24951.8189894E-117.54503251
CTCAAAT11200.00154689937.1110381
CTCCAAT13802.662632E-46.8206011
CAAGAAC16354.2969405E-56.6425041
TTTGATC12050.00346414166.58696462
CTTGAAT14755.9898524E-46.38130761
CTCTTTA15952.2320653E-46.35514831
CTTTAAT20704.170228E-66.29593941
CTATAAT15158.278204E-46.21282431
CCCAAAG13050.0076703976.10296061
CTTCAAT24351.5000896E-65.9468831
CCTTCAC14750.00416324575.8904381
TCTTATT21304.558207E-55.75902562
CCTTGTC15250.00602718025.69730851