FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612921
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2209.875948E-725.5363399
CACACGT2251.2012806E-624.9688667
ACACGTC2604.2204338E-621.6076728
AGCACAC3901.3755854E-414.403945
GAGCACA3901.3755854E-414.403944
AGAGCAC4754.8821974E-513.3068633
ATGCGTT5100.001635413.144387145
GCACACG4403.8196542E-412.7681696
GAAGAGC8600.0012176228.1823791
GATTATG8150.00698726167.75426775
GTCACTC2700.0021809546.241707320-24
AGTCACT2500.00674989876.1792920-24