FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637351
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCTCGTATGCCGT7540.11830216003426684TruSeq Adapter, Index 13 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3400.044.3572857
CACGTCT4550.037.6689839
ACACGTC4500.036.5640268
AGCACAC5350.029.4710245
GAGCACA5550.027.1738324
GCACACG5900.025.5618256
AGAGCAC6000.025.1357963
AAGAGCA9750.015.4839732
GAAGAGC10750.013.4612851
CCCATTT4450.00692454710.8396111
CTCTTGC5200.001816624110.6013781
CTGGATT5300.00212858410.4013521
CTCACAT3151.8189894E-1210.01865230-34
AGTCTCA3700.09.65207825-29
AAACTTC5850.0050310669.3746565
AGTCACA4300.09.27567920-24
AGGTTGC6150.00758897378.9173566
TCTCACA3705.456968E-128.90023730-34
GTCTCAC3551.8189894E-118.88979130-34
CACAGTC4002.5465852E-118.24138925-29
Produced by FastQC (version 0.11.5)