FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418335
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT5250.001228492211.1121371
CTCCAAG8951.2896105E-48.9626521
ATCGACT11602.5175573E-47.54381
CCCAGAT12003.719262E-47.292341
CTCCGAA14053.0386983E-46.74736071
CTCAAAC10850.0083463786.72105071
ATTGCAT14604.8515247E-46.49317931
CTTTGAT18402.9119612E-56.34116551
CTTCAAT17409.208325E-56.28649951
CTCTTTT25504.7243884E-76.0054561
CAACGAA14650.00356378555.97324751
CTCAAAA18502.1333966E-45.9127081
AACAAGA20803.146837E-55.9006172
CAAAGGC17356.013834E-45.88430931
CTATTAT18602.2960496E-45.88091951
CTTTCAT22257.53261E-55.57167531
CTTTTAT25101.3950856E-55.52009731
CTTTAAA17400.0039687635.44831
CAACAAG20106.5569667E-45.44204471
CTCCCGG19450.00255560135.24898531