FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775888
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG6154.0548554E-410.6039241
CTCCCGA10357.216966E-58.4011761
CCCGCTT11450.00193818936.96123741
GGTCAAT15051.03831335E-46.7404791
CTCCGAT12800.0062278756.22704361
GTTTGCG12950.0070283116.1549161
CGAAGAT18452.2441233E-45.8910691
CTCCAAT18502.3284092E-45.87514731
CTCCATA21755.8829526E-55.6635521
CTCATCC17950.00100667035.65148831
TTGCGCA16600.00262149035.6427958
CCCCCCC3100.0076208465.578449290-94
CCACAAT15700.0082411445.53835541
CTTCAAT31401.37592E-75.53835541
CTCAAAG17400.00428045635.4136891
TCACATT19100.00235421055.2836882
CTTTAAT29306.882221E-65.19338941
CTTTGAC18250.00749036165.1615451
AGATTGT22556.432048E-45.11248976
GATTTCG18600.0099780715.0360435