FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657841
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5057.909846E-613.4597977
CTCGCTA3100.00895825613.1558689
CACGTCT6358.256356E-510.7042489
CTTAGAA6100.0074600198.9375421
AGCACAC10150.00106420677.3664215
GTTTAAA10800.00199824046.94107681
AGAGCAC9850.0064651346.9011253
CTTGTCT12650.00146751976.4647041
ATTTAAG11950.00592601636.25683456
TTGAATT22601.2175751E-66.01721572
CATTGAG15200.00183865085.8133935
ATTTACC16800.00600758245.25973656
ATCAAAT25806.77858E-55.0187071
Produced by FastQC (version 0.11.5)