FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060143
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGC3708.0117636E-515.3495666
AGCACAC5150.001308720811.0278455
ACACGTC5450.002097768510.4208078
GAGCACA5600.002626318310.1426354
CACGTCT5850.00377350729.7082749
GCACACG5900.00404837549.6259996
CACACGT5950.00434045739.5451097
GAAGAGC10250.00588597546.96776061
TCAAATC15450.0086075165.5160032
CTTTTGT17400.00506883955.33594371
Produced by FastQC (version 0.11.5)