FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117099
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAA7600.00307281088.4971951
GTTAGAA9950.00421818867.2114671
CTCTCAT11450.002149586.8934071
CTCCGAG10600.0077936896.7692541
CTCTTGA15300.00103992046.09675361
CTCTGTA13100.0087647276.0251531
CCAGACC17550.0042728615.8769503145
CTCAACA15250.00666327175.6462241
GTTAAAG15500.0079727145.5551561
CTTCAAA39056.6393113E-105.51247841
CGAACAC15700.0091808585.484391
ATTTACG18400.00164243465.43724166
CTAAAAT23402.0523847E-45.21290451
Produced by FastQC (version 0.11.5)