FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621993
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAAA13452.2555469E-1010.3332361
CTTCAAT10901.2253859E-69.3952111
CTCAAAA8054.2923234E-49.0867621
CAATTCC8901.1704874E-49.0408171
CCTTGAT7350.00190134068.956951
ATTTCGT6550.00747206438.9341591
CTCCTGT6600.0079527518.8664771
CTTCCAC8450.00669800357.79095751
CTTTTTT22205.293259E-107.57844161
CTTTCAA12555.9825135E-46.99427271
CTTTTCA13852.4478775E-46.8659181
CATCAAT10750.00739453876.80450531
CTTTTAT13450.00131460646.5262541
CAAAGAA18653.3758282E-56.2754691
TCAATAT14350.0031077456.0455863
CTTCTTT21402.461803E-45.46904131
TTCAAAA18800.00186060095.3819482
Produced by FastQC (version 0.11.5)