FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431491
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCT10104.839606E-58.6932621
CTCGTAT10658.9968795E-58.2443141
CTCACGA10104.5965696E-47.96882341
CTCAAAT22455.311449E-97.17016651
CTCCGAT16405.0717135E-67.138371
CTCTATG16505.552307E-67.0951071
AGTTTAG10200.0050337747.08173666
CTTCCGT24857.6215656E-107.06655551
CCCTGTT20807.215931E-87.03541231
CTTTAAG24454.0308805E-96.88290641
CTTCAAT37500.06.8290411
CTCAAGA18303.5315625E-66.79705431
CTCATGA14554.4719686E-46.5373731
CTTCAGA27255.5861165E-96.44418051
CTCACAA12500.00439796276.43880941
CCCGCTT17308.131356E-56.34407141
CCCAGCT19657.232871E-55.95772361
AATTGAG18252.0198623E-45.93701745
CTTCTTT85400.05.8260471
CTCCAAT19053.0402222E-45.7612831
Produced by FastQC (version 0.11.5)