FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350747
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC9151.8561911E-48.6680099
CCCTATA9552.9085105E-48.3153671
CTCCAAT34450.07.5440641
CTCTTGT36800.07.45466041
CCCAAAT22951.4242687E-97.23497871
CTCCAGT25452.3101165E-107.0916021
CTCGAAT20708.4586645E-86.97512051
CTCAGAT29602.0008883E-116.82902051
CTCAGAG15953.1774303E-56.78926371
CTCTGAA39400.06.77949861
CTTTGAT94700.06.70849041
CTTTGGA56500.06.51649141
CTTTGAA79800.06.5136091
CTCTATT34051.6370905E-116.36057331
CTCAATT31951.4006218E-106.326731
CTCCAGC37801.8189894E-126.30252031
CTAAGCT14907.025418E-46.29867461
CTCTGAT45100.06.24281071
CTTTCGA29004.305548E-96.2234921
CTATTGT36001.0913936E-116.2165771
Produced by FastQC (version 0.11.5)