FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857212
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCAC3300.00797731413.3877411
AACTGAG3300.0090389613.1369855
CCCTTAT5550.001800185310.6137051
CTACGAA4900.00865736410.518941
ATTGGAG6405.0442625E-410.3545811
CTTCTAT7802.931934E-49.4400741
CTTTGAA14801.1461452E-88.9553131
TCGCTTT8205.842116E-48.8113923
CTTTAAA11251.706388E-58.5086541
ATTTGCG7900.00408917538.2314026
CAACAAA13752.0688168E-46.9616251
ACGATTT10600.00727805546.81635953
CAAAAAA27302.2314089E-75.93376061
CGAAAAA18100.00562483265.28852751