FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957223
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACT2650.001947971116.5501881
CTTCCGT10303.2196112E-1012.0644991
CTCGGAG4957.3531136E-411.8136031
CCCAGAG4550.00527017411.2456411
CCCACTG4700.00670897210.8867381
CTCCCGG11051.2389137E-810.5841331
CTCCAGT7000.00122788939.3981431
CTCTGGT6400.0062189919.1370841
CTCCAAC6450.0066296059.0662541
AACGTTT6500.00779309878.8893613
CTCCAAT6700.0090547698.7279611
CTCAGGA6700.0090547698.7279611
CTCCGAT7550.00244452368.713511
CTTCCAT15854.174217E-88.3011991
CTCATAT8550.00749690927.69438651
CTTCTGA13201.370415E-47.19891451
CTTCTTT30800.07.11980531
CTTCGAT10350.00516177257.06248041
CTTTTAA15551.8653136E-57.0511251
ATATAAG10250.0052798357.0460776
Produced by FastQC (version 0.11.5)