FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804273
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGT3200.007372061313.5483393
TATACCG6153.1937E-511.7485077
CACATGA4450.00442160611.5131561
CCCCCAT4800.00776807710.67365551
CTTTAAC6304.6108416E-410.4558251
CTCTTGA5900.00314426289.9241731
AATTCAG8256.2021584E-48.7585215
ATATACC7900.0040863768.2319026
CTTTGAT15351.8670744E-67.6290071
CTTTTGA13001.1163677E-47.3190781
CTTCAAT13150.00101255256.67900661
CAAAGAA21257.6869946E-76.5441171
CTTTTAT15801.712542E-46.48525951
CTTCTTT27152.2521999E-75.93074461
TCAATAT17356.7483564E-45.83060073
CTCTTTT19353.72865E-45.67370371
CTTCTTC25501.7035492E-55.45343071
CTTTCTT24152.4350392E-45.15214631
Produced by FastQC (version 0.11.5)