FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553303
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGT3250.007615084813.48096751
CTTCAGA6302.6148919E-612.7498571
CTCTTAT4600.00575555111.1120291
TCTCTGT5700.0026599510.1255129
CTTTCAT9151.5397507E-59.5766431
CTCAAAA7803.1828583E-49.3617831
CTTCGAC6500.00711506238.9873111
TCAGGAT7950.00437564268.1687532
CTTGAAG11502.235361E-47.6196771
TTCGAAT2650.00119412316.584037125-129
AAATATT15800.0092231825.48030234
AAAATAT19000.00216672485.31685453
Produced by FastQC (version 0.11.5)