FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774372
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCT12651.0492437E-58.0158861
CTAATAT10851.2567817E-48.010611
CCCAGGA12407.0946815E-57.593391
CCCAGCT18801.09912435E-77.31997871
CGAAGAT21251.6983904E-87.15771531
ATAAGGC13201.6386049E-47.0961882
CCCTGCA18607.146591E-77.00928351
CTCAAAT14404.4582682E-46.5387531
TATAACA16955.22372E-45.9496537
CCCGAAA20802.997403E-55.91969971
CAACGAT15100.0053832795.75596761
CTTTGAA29101.759854E-75.7246491
CTTCAAA28456.8664485E-75.6008571
TCAAAGT16750.00291700755.59222032
CAACTCC15600.00771705765.57148171
CCCTTCT15650.0079940615.55368141
CCCTTGC18700.00169901245.42251161
CTCCAAA20408.766049E-45.3256811
CTTTGGA17850.00580807125.27496051
CTAAAAA20609.986977E-45.2739751