FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546165
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1558.3127816E-1041.593537
CACGTCT2152.0298103E-829.9887779
ACACGTC2901.3489625E-824.7033988
GCACACG3356.225491E-821.385036
GAGCACA3707.612471E-921.2983364
AGAGCAC3754.3208584E-617.1935653
AGCACAC3855.551128E-616.7469795
TTGGAGG4200.003361574611.94216252
CTTCGGA5300.001499978410.8473311
CTTGCCA6050.00449725759.502621
AAAAGTT8901.4674019E-48.8543644
AAGAGCA8408.056585E-48.5301162
CCAAAAA7600.00302331368.5101591
GAAGAGC8000.00480267368.0846511
CAAAGGA8150.00567545287.93585351
TGACGTG2050.00565997326.996813325-29
TTCCGCC11650.00260929676.76551682
CTCGTAT2800.00257936386.14900435-39
CCGCTTA3150.0014822025.91952625-29
ACGTCTG3200.00181252715.820738310-14
Produced by FastQC (version 0.11.5)