FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601144
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAT5256.519538E-613.7106671
CCCACAG3750.001386755313.4364531
CTTCAAG7251.7632409E-49.9284131
CCCATTT8155.701072E-48.8320251
GTCCTTT6550.0085245718.7915731
CCCAAAG6550.0085245718.7915731
CACAAAG8256.437738E-48.724971
CTTTAAT9904.4059768E-47.99788951
TTAATAT10650.0083698336.7177363
CATTGAT12909.82739E-46.6959071
CTTTTAT13450.00157642636.42209671
ATTTTCT19250.00284525035.2027596
Produced by FastQC (version 0.11.5)