FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179773
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAG2750.002696894315.7636041
CTCGTTT8153.5396806E-711.5245171
GGTCTAT5902.8361715E-411.0211641
CTCGCTT6657.094895E-510.864641
CAACAAC14551.1878001E-99.4346891
CTTAAAC6300.00601492179.1745841
CTCAAAT10609.854401E-58.1792291
CAACGGA9753.599639E-48.1512651
CAACGAT10751.16053816E-48.06511
CTTCTAT16005.1605457E-77.6765471
ATTGTGC9400.00228882377.6760558
CTTATGG8600.0087681347.56103131
CCCATCT8600.0087681347.56103131
CTCCGAA10600.0072863076.8160241
CTCTGAT10800.0087277686.68980071
CCTGAAT11950.00313113846.65061331
CTCCGGT13450.0015150446.4460841
CTTCAAT17901.5425481E-46.05445671
CTTTAAA19105.742874E-56.05234341
GTTTTGC16904.9007003E-45.9788516
Produced by FastQC (version 0.11.5)